Publication list - Publika - Scientific publications from Oslo University Hospital

Publications by Pål Øystein Falnes

71 publications found

Original articles

Willemen HLDM, Santos Ribeiro PS, Broeks M, Meijer N, Versteeg S, Tiggeler A, de Boer TP, Małecki JM, Falnes PØ, Jans J, Eijkelkamp N (2023)
Inflammation-induced mitochondrial and metabolic disturbances in sensory neurons control the switch from acute to chronic pain
Cell Rep Med, 4 (11), 101265
DOI 10.1016/j.xcrm.2023.101265, PubMed 37944527

Engelfriet ML, Małecki JM, Forsberg AF, Falnes PØ, Ciosk R (2023)
Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans
PLoS One, 18 (6), e0287558
DOI 10.1371/journal.pone.0287558, PubMed 37347777

Małecki JM, Odonohue MF, Kim Y, Jakobsson ME, Gessa L, Pinto R, Wu J, Davydova E, Moen A, Olsen JV, Thiede B, Gleizes PE, Leidel SA, Falnes PØ (2021)
Human METTL18 is a histidine-specific methyltransferase that targets RPL3 and affects ribosome biogenesis and function
Nucleic Acids Res, 49 (6), 3185-3203
DOI 10.1093/nar/gkab088, PubMed 33693809

Davydova E, Shimazu T, Schuhmacher MK, Jakobsson ME, Willemen HLDM, Liu T, Moen A, Ho AYY, Małecki J, Schroer L, Pinto R, Suzuki T, Grønsberg IA, Sohtome Y, Akakabe M, Weirich S, Kikuchi M, Olsen JV, Dohmae N, Umehara T, Sodeoka M, Siino V, McDonough MA, Eijkelkamp N, Schofield CJ et al. (2021)
The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes
Nat Commun, 12 (1), 891
DOI 10.1038/s41467-020-20670-7, PubMed 33563959

Pinto R, Vågbø CB, Jakobsson ME, Kim Y, Baltissen MP, O'Donohue MF, Guzmán UH, Małecki JM, Wu J, Kirpekar F, Olsen JV, Gleizes PE, Vermeulen M, Leidel SA, Slupphaug G, Falnes PØ (2020)
The human methyltransferase ZCCHC4 catalyses N6-methyladenosine modification of 28S ribosomal RNA
Nucleic Acids Res, 48 (2), 830-846
DOI 10.1093/nar/gkz1147, PubMed 31799605

Małecki JM, Willemen HLDM, Pinto R, Ho AYY, Moen A, Eijkelkamp N, Falnes PØ (2019)
Human FAM173A is a mitochondrial lysine-specific methyltransferase that targets adenine nucleotide translocase and affects mitochondrial respiration
J Biol Chem, 294 (31), 11654-11664
DOI 10.1074/jbc.RA119.009045, PubMed 31213526

Małecki JM, Willemen HLDM, Pinto R, Ho AYY, Moen A, Kjønstad IF, Burgering BMT, Zwartkruis F, Eijkelkamp N, Falnes PØ (2018)
Lysine methylation by the mitochondrial methyltransferase FAM173B optimizes the function of mitochondrial ATP synthase
J Biol Chem, 294 (4), 1128-1141
DOI 10.1074/jbc.RA118.005473, PubMed 30530489

Jakobsson ME, Małecki JM, Halabelian L, Nilges BS, Pinto R, Kudithipudi S, Munk S, Davydova E, Zuhairi FR, Arrowsmith CH, Jeltsch A, Leidel SA, Olsen JV, Falnes PØ (2018)
The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates
Nat Commun, 9 (1), 3411
DOI 10.1038/s41467-018-05646-y, PubMed 30143613

Jakobsson ME, Małecki J, Falnes PØ (2018)
Regulation of eukaryotic elongation factor 1 alpha (eEF1A) by dynamic lysine methylation
RNA Biol, 15 (3), 314-319
DOI 10.1080/15476286.2018.1440875, PubMed 29447067

Willemen HLDM, Kavelaars A, Prado J, Maas M, Versteeg S, Nellissen LJJ, Tromp J, Gonzalez Cano R, Zhou W, Jakobsson ME, Małecki J, Posthuma G, Habib AM, Heijnen CJ, Falnes PØ, Eijkelkamp N (2018)
Identification of FAM173B as a protein methyltransferase promoting chronic pain
PLoS Biol, 16 (2), e2003452
DOI 10.1371/journal.pbio.2003452, PubMed 29444090

Małecki J, Jakobsson ME, Ho AYY, Moen A, Rustan AC, Falnes PØ (2017)
Uncovering human METTL12 as a mitochondrial methyltransferase that modulates citrate synthase activity through metabolite-sensitive lysine methylation
J Biol Chem, 292 (43), 17950-17962
DOI 10.1074/jbc.M117.808451, PubMed 28887308

Jakobsson ME, Malecki J, Nilges BS, Moen A, Leidel SA, Falnes PØ (2017)
Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation
Nucleic Acids Res, 45 (14), 8239-8254
DOI 10.1093/nar/gkx432, PubMed 28520920

Malecki J, Aileni VK, Ho AYY, Schwarz J, Moen A, Sørensen V, Nilges BS, Jakobsson ME, Leidel SA, Falnes PØ (2017)
The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A)
Nucleic Acids Res, 45 (8), 4370-4389
DOI 10.1093/nar/gkx002, PubMed 28108655

Aileni VK, Davydova E, Moen A, Falnes PØ (2017)
A System for Enzymatic Lysine Methylation in a Desired Sequence Context
ACS Omega, 2 (2), 462-469
DOI 10.1021/acsomega.6b00486, PubMed 28357416

Małecki J, Dahl HA, Moen A, Davydova E, Falnes PØ (2016)
The METTL20 Homologue from Agrobacterium tumefaciens Is a Dual Specificity Protein-lysine Methyltransferase That Targets Ribosomal Protein L7/L12 and the β Subunit of Electron Transfer Flavoprotein (ETFβ)
J Biol Chem, 291 (18), 9581-95
DOI 10.1074/jbc.M115.709261, PubMed 26929405

Fusser M, Kernstock S, Aileni VK, Egge-Jacobsen W, Falnes PØ, Klungland A (2015)
Lysine Methylation of the Valosin-Containing Protein (VCP) Is Dispensable for Development and Survival of Mice
PLoS One, 10 (11), e0141472
DOI 10.1371/journal.pone.0141472, PubMed 26544960

Jakobsson ME, Moen A, Davidson B, Falnes PØ (2015)
Hsp70 (HSPA1) Lysine Methylation Status as a Potential Prognostic Factor in Metastatic High-Grade Serous Carcinoma
PLoS One, 10 (10), e0140168
DOI 10.1371/journal.pone.0140168, PubMed 26448330

Jakobsson ME, Davydova E, Małecki J, Moen A, Falnes PØ (2015)
Saccharomyces cerevisiae Eukaryotic Elongation Factor 1A (eEF1A) Is Methylated at Lys-390 by a METTL21-Like Methyltransferase
PLoS One, 10 (6), e0131426
DOI 10.1371/journal.pone.0131426, PubMed 26115316

Zdżalik D, Domańska A, Prorok P, Kosicki K, van den Born E, Falnes PØ, Rizzo CJ, Guengerich FP, Tudek B (2015)
Differential repair of etheno-DNA adducts by bacterial and human AlkB proteins
DNA Repair (Amst), 30, 1-10
DOI 10.1016/j.dnarep.2015.02.021, PubMed 25797601

Małecki J, Ho AY, Moen A, Dahl HA, Falnes PØ (2014)
Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity
J Biol Chem, 290 (1), 423-34
DOI 10.1074/jbc.M114.614115, PubMed 25416781

Davydova E, Ho AYY, Malecki J, Moen A, Enserink JM, Jakobsson ME, Loenarz C, Falnes PØ (2014)
Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2)
J Biol Chem, 289 (44), 30499-30510
DOI 10.1074/jbc.M114.601658, PubMed 25231979

Zdżalik D, Vågbø CB, Kirpekar F, Davydova E, Puścian A, Maciejewska AM, Krokan HE, Klungland A, Tudek B, van den Born E, Falnes PØ (2014)
Protozoan ALKBH8 oxygenases display both DNA repair and tRNA modification activities
PLoS One, 9 (6), e98729
DOI 10.1371/journal.pone.0098729, PubMed 24914785

Jakobsson ME, Moen A, Bousset L, Egge-Jacobsen W, Kernstock S, Melki R, Falnes PØ (2013)
Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation
J Biol Chem, 288 (39), 27752-63
DOI 10.1074/jbc.M113.483248, PubMed 23921388

Lin FJ, Shen L, Jang CW, Falnes PØ, Zhang Y (2013)
Ikbkap/Elp1 deficiency causes male infertility by disrupting meiotic progression
PLoS Genet, 9 (5), e1003516
DOI 10.1371/journal.pgen.1003516, PubMed 23717213

Meza TJ, Moen MN, Vågbø CB, Krokan HE, Klungland A, Grini PE, Falnes PØ (2012)
The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana against methylation damage
Nucleic Acids Res, 40 (14), 6620-31
DOI 10.1093/nar/gks327, PubMed 22532610

Korvald H, Falnes PØ, Laerdahl JK, Bjørås M, Alseth I (2012)
The Schizosaccharomyces pombe AlkB homolog Abh1 exhibits AP lyase activity but no demethylase activity
DNA Repair (Amst), 11 (5), 453-62
DOI 10.1016/j.dnarep.2012.01.014, PubMed 22365419

Kernstock S, Davydova E, Jakobsson M, Moen A, Pettersen S, Mælandsmo GM, Egge-Jacobsen W, Falnes PØ (2012)
Lysine methylation of VCP by a member of a novel human protein methyltransferase family
Nat Commun, 3, 1038
DOI 10.1038/ncomms2041, PubMed 22948820

Bjørnstad LG, Meza TJ, Otterlei M, Olafsrud SM, Meza-Zepeda LA, Falnes PØ (2012)
Human ALKBH4 interacts with proteins associated with transcription
PLoS One, 7 (11), e49045
DOI 10.1371/journal.pone.0049045, PubMed 23145062

Leihne V, Kirpekar F, Vågbø CB, van den Born E, Krokan HE, Grini PE, Meza TJ, Falnes PØ (2011)
Roles of Trm9- and ALKBH8-like proteins in the formation of modified wobble uridines in Arabidopsis tRNA
Nucleic Acids Res, 39 (17), 7688-701
DOI 10.1093/nar/gkr406, PubMed 21653555

Bjørnstad LG, Zoppellaro G, Tomter AB, Falnes PØ, Andersson KK (2011)
Spectroscopic and magnetic studies of wild-type and mutant forms of the Fe(II)- and 2-oxoglutarate-dependent decarboxylase ALKBH4
Biochem J, 434 (3), 391-8
DOI 10.1042/BJ20101667, PubMed 21166655

van den Born E, Vågbø CB, Songe-Møller L, Leihne V, Lien GF, Leszczynska G, Malkiewicz A, Krokan HE, Kirpekar F, Klungland A, Falnes PØ (2011)
ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA
Nat Commun, 2, 172
DOI 10.1038/ncomms1173, PubMed 21285950

Songe-Møller L, van den Born E, Leihne V, Vågbø CB, Kristoffersen T, Krokan HE, Kirpekar F, Falnes PØ, Klungland A (2010)
Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding
Mol Cell Biol, 30 (7), 1814-27
DOI 10.1128/MCB.01602-09, PubMed 20123966

van den Born E, Bekkelund A, Moen MN, Omelchenko MV, Klungland A, Falnes PØ (2009)
Bioinformatics and functional analysis define four distinct groups of AlkB DNA-dioxygenases in bacteria
Nucleic Acids Res, 37 (21), 7124-36
DOI 10.1093/nar/gkp774, PubMed 19786499

van den Born E, Omelchenko MV, Bekkelund A, Leihne V, Koonin EV, Dolja VV, Falnes PØ (2008)
Viral AlkB proteins repair RNA damage by oxidative demethylation
Nucleic Acids Res, 36 (17), 5451-61
DOI 10.1093/nar/gkn519, PubMed 18718927

Ringvoll J, Moen MN, Nordstrand LM, Meira LB, Pang B, Bekkelund A, Dedon PC, Bjelland S, Samson LD, Falnes PØ, Klungland A (2008)
AlkB homologue 2-mediated repair of ethenoadenine lesions in mammalian DNA
Cancer Res, 68 (11), 4142-9
DOI 10.1158/0008-5472.CAN-08-0796, PubMed 18519673

Falnes PØ, Klungland A, Alseth I (2006)
Repair of methyl lesions in DNA and RNA by oxidative demethylation
Neuroscience, 145 (4), 1222-32
DOI 10.1016/j.neuroscience.2006.11.018, PubMed 17175108

Ringvoll J, Nordstrand LM, Vågbø CB, Talstad V, Reite K, Aas PA, Lauritzen KH, Liabakk NB, Bjørk A, Doughty RW, Falnes PØ, Krokan HE, Klungland A (2006)
Repair deficient mice reveal mABH2 as the primary oxidative demethylase for repairing 1meA and 3meC lesions in DNA
EMBO J, 25 (10), 2189-98
DOI 10.1038/sj.emboj.7601109, PubMed 16642038

Falnes PØ (2005)
RNA repair--the latest addition to the toolbox for macromolecular maintenance
RNA Biol, 2 (1), 14-6
DOI 10.4161/rna.2.1.1602, PubMed 17132930

Ougland R, Zhang CM, Liiv A, Johansen RF, Seeberg E, Hou YM, Remme J, Falnes PØ (2004)
AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation
Mol Cell, 16 (1), 107-16
DOI 10.1016/j.molcel.2004.09.002, PubMed 15469826

Aamodt RM, Falnes PØ, Johansen RF, Seeberg E, Bjørås M (2004)
The Bacillus subtilis counterpart of the mammalian 3-methyladenine DNA glycosylase has hypoxanthine and 1,N6-ethenoadenine as preferred substrates
J Biol Chem, 279 (14), 13601-6
DOI 10.1074/jbc.M314277200, PubMed 14729667

Falnes PØ (2004)
Repair of 3-methylthymine and 1-methylguanine lesions by bacterial and human AlkB proteins
Nucleic Acids Res, 32 (21), 6260-7
DOI 10.1093/nar/gkh964, PubMed 15576352

Falnes PØ, Bjørås M, Aas PA, Sundheim O, Seeberg E (2004)
Substrate specificities of bacterial and human AlkB proteins
Nucleic Acids Res, 32 (11), 3456-61
DOI 10.1093/nar/gkh655, PubMed 15229293

Aas PA, Otterlei M, Falnes PO, Vågbø CB, Skorpen F, Akbari M, Sundheim O, Bjørås M, Slupphaug G, Seeberg E, Krokan HE (2003)
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
Nature, 421 (6925), 859-63
DOI 10.1038/nature01363, PubMed 12594517

Falnes PØ, Johansen RF, Seeberg E (2002)
AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
Nature, 419 (6903), 178-82
DOI 10.1038/nature01048, PubMed 12226668

Wesche J, Wiedłocha A, Falnes PO, Choe S, Olsnes S (2000)
Externally added aFGF mutants do not require extensive unfolding for transport to the cytosol and the nucleus in NIH/3T3 cells
Biochemistry, 39 (49), 15091-100
DOI 10.1021/bi001831k, PubMed 11106487

Falnes PO (2000)
Design of toxins that can be activated by cell-specific proteases and their potential use in targeted cell killing
Int J Med Microbiol, 290 (4-5), 471-6
DOI 10.1016/S1438-4221(00)80068-5, PubMed 11111928

Falnes PO, Ariansen S, Sandvig K, Olsnes S (2000)
Requirement for prolonged action in the cytosol for optimal protein synthesis inhibition by diphtheria toxin
J Biol Chem, 275 (6), 4363-8
DOI 10.1074/jbc.275.6.4363, PubMed 10660606

Falnes PO, Welker R, Kräusslich HG, Olsnes S (1999)
Toxins that are activated by HIV type-1 protease through removal of a signal for degradation by the N-end-rule pathway
Biochem J, 343 Pt 1 (Pt 1), 199-207
DOI 10.1042/0264-6021:3430199, PubMed 10493930

Kolpakova E, Wiedłocha A, Stenmark H, Klingenberg O, Falnes PO, Olsnes S (1998)
Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor
Biochem J, 336 ( Pt 1) (Pt 1), 213-22
DOI 10.1042/bj3360213, PubMed 9806903

Wesche J, Elliott JL, Falnes PO, Olsnes S, Collier RJ (1998)
Characterization of membrane translocation by anthrax protective antigen
Biochemistry, 37 (45), 15737-46
DOI 10.1021/bi981436i, PubMed 9843379

Klingenberg O, Widlocha A, Rapak A, Muñoz R, Falnes P, Olsnes S (1998)
Inability of the acidic fibroblast growth factor mutant K132E to stimulate DNA synthesis after translocation into cells
J Biol Chem, 273 (18), 11164-72
DOI 10.1074/jbc.273.18.11164, PubMed 9556604

Falnes PO, Olsnes S (1998)
Modulation of the intracellular stability and toxicity of diphtheria toxin through degradation by the N-end rule pathway
EMBO J, 17 (2), 615-25
DOI 10.1093/emboj/17.2.615, PubMed 9430652

Muñoz R, Klingenberg O, Wiedłocha A, Rapak A, Falnes PO, Olsnes S (1997)
Effect of mutation of cytoplasmic receptor domain and of genistein on transport of acidic fibroblast growth factor into cells
Oncogene, 15 (5), 525-36
DOI 10.1038/sj.onc.1201226, PubMed 9247306

Rapak A, Falnes PO, Olsnes S (1997)
Retrograde transport of mutant ricin to the endoplasmic reticulum with subsequent translocation to cytosol
Proc Natl Acad Sci U S A, 94 (8), 3783-8
DOI 10.1073/pnas.94.8.3783, PubMed 9108055

Lanzrein M, Falnes PO, Sand O, Olsnes S (1997)
Structure-function relationship of the ion channel formed by diphtheria toxin in Vero cell membranes
J Membr Biol, 156 (2), 141-8
DOI 10.1007/s002329900196, PubMed 9075645

Wiedłocha A, Falnes PO, Rapak A, Muñoz R, Klingenberg O, Olsnes S (1996)
Stimulation of proliferation of a human osteosarcoma cell line by exogenous acidic fibroblast growth factor requires both activation of receptor tyrosine kinase and growth factor internalization
Mol Cell Biol, 16 (1), 270-80
DOI 10.1128/MCB.16.1.270, PubMed 8524304

Wiedłocha A, Falnes PO, Rapak A, Klingenberg O, Muñoz R, Olsnes S (1995)
Translocation of cytosol of exogenous, CAAX-tagged acidic fibroblast growth factor
J Biol Chem, 270 (51), 30680-5
DOI 10.1074/jbc.270.51.30680, PubMed 8530506

Falnes PO, Wiedłocha A, Rapak A, Olsnes S (1995)
Farnesylation of CaaX-tagged diphtheria toxin A-fragment as a measure of transfer to the cytosol
Biochemistry, 34 (35), 11152-9
DOI 10.1021/bi00035a021, PubMed 7669773

Falnes PO, Olsnes S (1995)
Cell-mediated reduction and incomplete membrane translocation of diphtheria toxin mutants with internal disulfides in the A fragment
J Biol Chem, 270 (35), 20787-93
DOI 10.1074/jbc.270.35.20787, PubMed 7657662

Wiedłocha A, Falnes PO, Madshus IH, Sandvig K, Olsnes S (1994)
Dual mode of signal transduction by externally added acidic fibroblast growth factor
Cell, 76 (6), 1039-51
DOI 10.1016/0092-8674(94)90381-6, PubMed 7511061

Falnes PO, Choe S, Madshus IH, Wilson BA, Olsnes S (1994)
Inhibition of membrane translocation of diphtheria toxin A-fragment by internal disulfide bridges
J Biol Chem, 269 (11), 8402-7
PubMed 8132565

Esbensen QY, Falnes PO, Olsnes S, Madshus IH (1993)
Subcloning and characterization of the binding domain of fragment B of diphtheria toxin
Biochem J, 294 ( Pt 3) (Pt 3), 663-6
DOI 10.1042/bj2940663, PubMed 8379922

Falnes PO, Madshus IH, Sandvig K, Olsnes S (1992)
Replacement of negative by positive charges in the presumed membrane-inserted part of diphtheria toxin B fragment. Effect on membrane translocation and on formation of cation channels
J Biol Chem, 267 (17), 12284-90
PubMed 1376320

Review articles

Falnes PØ, Małecki JM, Herrera MC, Bengtsen M, Davydova E (2023)
Human seven-β-strand (METTL) methyltransferases - conquering the universe of protein lysine methylation
J Biol Chem, 299 (6), 104661
DOI 10.1016/j.jbc.2023.104661, PubMed 36997089

Małecki JM, Davydova E, Falnes PØ (2022)
Protein methylation in mitochondria
J Biol Chem, 298 (4), 101791
DOI 10.1016/j.jbc.2022.101791, PubMed 35247388

Falnes PØ, Jakobsson ME, Davydova E, Ho A, Małecki J (2016)
Protein lysine methylation by seven-β-strand methyltransferases
Biochem J, 473 (14), 1995-2009
DOI 10.1042/BCJ20160117, PubMed 27407169

Falnes PØ, Rognes T (2003)
DNA repair by bacterial AlkB proteins
Res Microbiol, 154 (8), 531-8
DOI 10.1016/S0923-2508(03)00150-5, PubMed 14527653

Falnes PO, Sandvig K (2000)
Penetration of protein toxins into cells
Curr Opin Cell Biol, 12 (4), 407-13
DOI 10.1016/s0955-0674(00)00109-5, PubMed 10873820